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Afterward, labeled secondary IRDye 800 CW Goat Anti Mouse antibody (926-32210 LI-COR Biosciences, 1:3000 dilution in Odyssey Blocking buffer) and Cell Tag 700 Stain (926-41090, LI-COR Biosciences, 1:4000) were added to each well and after 1 h, cells were washed four times with PBS containing 0.1% Tween-20