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If normal detoxification has not been occurring, then the sudden influx of large amounts of glutathione can cause rapid symptomatic detoxification, so starting the treatment at a lower dose and working up is best
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For Western blotting, all proteins were isolated using the RIPA buffer (Beyotime Institute of Biotechnology, Shanghai, China) and analyzed with bicinchoninic acid protein concentration assay kit (Beyotime Institute of Biotechnology, Shanghai, China)
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