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The hypothesis behind the LMR is that the smaller mannitol (6.7 Å) can pass via the pore pathway and can be considered as a marker for the total small intestinal surface, while the larger disaccharide lactulose (9.5 Å) will only pass the larger pores of the leak pathway in the crypt region and at sites where the barrier is damaged ( ex vivo in Ussing chambers, the difference is much less pronounced with a LMR of ~0.8 ex vivo vs