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For live-cell confocal microscopy, cells were visualized with a 100×/1.46 oil immersion objective using the Zeiss LSM 510 confocal microscope equipped with a TempModuleS and CO 2 Module (Zeiss) to maintain the temperature and CO 2 levels at 37 °C and 5.0%, respectively

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Conclusion In this paper, we reviewed the task of automated clinical coding from the perspectives of AI researchers and clinical coding professionals, what it is and why it is an important task, and summarised the challenges of the recent deep learning methods for the task