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For example, it is very difficult to target O 2 •− specifically (e.g., manipulations of O 2 •− levels will also affect H 2 O 2 ), but many more tools are available to modify steady-state levels of H 2 O 2 , such as exposure of culture cells to H 2 O 2 directly or treatment with the antioxidant N -acetyl cysteine, which impacts mainly H 2 O 2