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any of the seven commonly recognized stages thereof may be treated, symptoms thereof reversed or arrested without progression to a more severe stage, and/or the progression to more severe stages may be slowed

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Then 20 ml Microfil (MV-112, Flow Tech, Inc., Carver, MA, USA) was injected slowly at a uniform speed using a computer-controlled pneumatic pumps (Harvard PHD 2000) to ensure pressure ≤180 mmHg, and rats were sacrificed during this process