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Shotgun Mass Spectrometry Proteomics Cell lysates were reduced using 2.5 μL of dithiothreitol (200 mM DTT stock solution) (Sigma) at 90 °C for 20 min, and alkylated with 10 μL of Cysteine Blocking Reagent (Iodoacetamide, IAM, 200 mM Sigma) for 1 h at room temperature in the dark