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Absorbance titration experiments were recorded at 235-800 nm with the constant concentration of the HAS (2.0 x 10 -6 M), while the concentrations of α-methylbutyrylshikon ( 1 ), acetylshikonin ( 2 ) and β -hydroxyisovalerylshikonin ( 3 ) varied from 0 to 1.6 x 10 -5 M

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Dima et al., 2009)