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The membranes were then treated overnight at 4°C with the primary antibodies for rabbit polyclonal anti-PK2 (Abcam, MA, United States, Cat# ab87360, dilution 1: 1,000), rabbit polyclonal anti-PKR1 (BT LAB, Shanghai, China, Cat# BT-AP12193, dilution 1: 1,000), and mouse monoclonal anti-B-actin (Abcam, Cat# ab227387, dilution 1:2000)