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General procedure for dose-dependent CuNiP modification of lysates and conjugation with Cy5 azide fluorophore To 4 tubes (individual reactions) of 100 µg of lysate in degassed MeCN:H 2 O (1:4, 400 µL) were treated with freshly prepared 50 µM, 100 µM, 150 µM, and 200 µM CuBr that had been re-suspended in 50 µL of acetonitrile

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