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This was an in vitro study
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Arrestin 2 could regulate post-endocytic sorting of MOR upon DAMGO exposure but not morphine by enabling receptor ubiquitination, as described for different GPCRs (Marchese and Trejo, 2013), but also dephosphorylation on the S375 (Groer et al., 5 ]-enkephalin exposure (Arttamangkul et al., 5 ]-enkephalin- or morphine-induced acute desensitization but not cellular tolerance involves dephosphorylation mediated by protein phosphatases sensitive to calyculin A but not okadaic acid (Levitt and Williams, 2012)
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