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In addition, some of the assays involve the determination of HTL after on-column or post-column derivatization with o -phthaldialdehyde (OPA) 9,10,11 , while the other approaches employ pre-column derivatization of HTL with N -trimethylsilyl- N -methyl trifluoroacetamide 15 and isobutyl chloroformate 16

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