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Extraction and analyses of leaves essential oils After cutting fresh leaves from the plants, hydrodistilling the essential oil by a standard Clevenger, and diluting the hydrodistilled essential oil to 0.5% with n-hexane having HPLC-grade, the essential oil was injected into a Gas chromatography-mass spectrometry (GC–MS) system using an Agilent 7890B gas chromatograph equipped with a capillary column (Agilent HP-5MS, 30 m × 0.25 mm, coating thickness 0.25 μm) and a mass detector (Agilent 5977B)
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