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B12 injection treatment advice While your doctor may prescribe a specific regimen that suits your exact medical needs, the guideline recommended treatment for peripheral neuropathy using vitamin B12 injections is as follows: Daily injections of 1,000mcg for at least five days Followed by 1,000mcg injections administered once per week, for a period of five weeks Eventually, injections may be reduced to just 1000mcg, once a month, but you may be required to administer them for the rest of your life Most injection regimens are tailored to the individual since people absorb and utilize the vitamin differently