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By utilizing pooled CRISPR-Cas9 libraries that target every gene for knockout (CRISPRko), transcriptional inhibition (CRISPRi), or activation (CRISPRa), researchers can subject a population of cancer cells to a pro-ferroptotic agent like erastin or RSL3 and subsequently identify which genetic perturbations are enriched or depleted in the surviving cell population via next-generation sequencing

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The different pathological makers of various neurodegenerative diseases e.g., Lewy bodies, plaques, etc., indicate different causal factors in the initiation of these diseases