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For immunohistochemistry evaluation, hippocampal images (six images per group) of subregion cornu ammonis 1 (CA1), cornu ammonis 2 (CA2), cornu ammonis 3 (CA3) and dentate gyrus (DG) were captured at 40x magnification using a Ni-U upright microscope (Nikon Corporation) with NIS Element Imaging Software version 5 (Nikon Corporation)

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2019;7(1) doi: 10.1186/s40168-019-0713-7