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In patients with mild preeclampsia, maternal blood α-tocopherol concentrations were not decreased as compared with normal pregnancies (29,30), but in patients with severe preeclampsia plasma α-tocopherol was significantly decreased as compared with controls, which is thought to be caused by the fact that antioxidants may be utilized to a greater extent to counteract free radical-mediated cell disturbances, resulting in a reduction in their plasma levels (31)

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The extracted DNA concentration was measured with a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA)