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In brief, labeled peptide was diluted to 1 μM in artificial hemolymph and added at a 1:10 ratio to the mounted tissues (final concentration 100 nM), and bound peptide was imaged using an LSM-900 confocal microscope (Zeiss) in AiryScan2 super-resolution mode with a 40x objective and GFP and BODIPY-543 filter settings

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doi: 10.1089/thy.2007.0040 [DOI] [PubMed] [Google Scholar] 52