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1 Subject areas: Biophysics, Protein Biochemistry, Structural Biology Graphical abstract Highlights • CD spectrometer can detect structural helicity in peptidomimetics • DMSO hinders CD detection when used to solubilize peptidomimetics • Instructions for removing DMSO from peptidomimetics using an evaporation assay • Reconstitute peptidomimetics in aqueous solutions for optimal CD detection Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics