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Some 50 µL of the homogenate was then transferred to a new 1.5-mL Eppendorf tube, diluted with 450 µL of distilled water, and mixed with 50 µL of ice-cold, 10× TCA solution (72% trichloroacetic acid in water)

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Risk of renal injury In addition to CVD and GI complications, NSAIDs also carry a significant risk of kidney damage [171] which includes multiple nephrological complications like acute kidney injury (AKI), and chronic kidney disease (CKD) encompassing electrolyte imbalance, glomerulonephritis, renal papillary necrosis, fluid retention-induced hypertension, renal tubular acidosis, hyponatremia and hyperkalemia to name a few [172], [173]