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EpSCs organoid (3D) culture EpSCs were plated in 80% Matrigel (Corning, 356231) in EpSCs organoid culture media (Advance DMEM/F12 (Life Technologies, 12634028), 2 mM glutamine (Sigma-Aldrich, 90114 C), 50 μg/ml penicillin and streptomycin (Sigma-Aldrich, H3375), 50 ng/ml mEGF (R&D Systems, 2028-WG-200), 500 ng/ml R-spondin (R&D Systems, 3474-RS), 100 ng/ml Noggin (PeproTech, 250-38-250ug), 1X B-27 (Fisher Scientific, 11530536), 10 ng/ml Forskolin (Tocris, 1099), 10 µM Y27632 (BD Biosciences, 562822)
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