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Cells were cultured with RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin, and then incubated in a humidified atmosphere containing 5% CO 2 at 37°C

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NAD+ Preservation and Sirtuin Activation First and most directly, NNMT activity consumes nicotinamide, which is a key precursor for NAD+ biosynthesis through the salvage pathway (the predominant NAD+ synthesis route in most mammalian cells)

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