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OT-I T-cells were gated using CD45 + (Clone-30-F11), CD3 + (Clone-17A2), CD8 + (Clone-53-6.7), CD4 - (Clone-GK1.5), CD44 + (Clone- IM7), CD62L - (Clone-MEL-14), all purchased from Biolegend, and analyzed using monoclonal antibodies specific to: CXCR3 (Clone- FAB1685P), CCR4 (Clone-2G12), CXCR4 (clone- L276F12), α4 (CD49, Clone-9C10), β1 (CD29 Clone- HMb1-1), β7 (CD80 Clone- FIB504), αL (CD11a, clone- M17/4), β2 (CD18, Clone- M18/2), CD103 (Clone-2E7) and CXCR6 (Clone-SA051D1), all from Biolegend
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